The Screening of Biopolymer Bacteria in Porous MediaThe Screening of Biopolymer Bacteria in Porous Media

Abstract

MEOR has advantages of less polluting environment and low cost, which can be extended to the volume of the biopolymer produced by metabolism, and it is one of the main mechanisms of microbial enhanced oil recovery. The fermentation, the study of biopolymers produced growth and metabolism of bacteria rules; after using the orthogonal experimental method and optimized the optimum culture conditions, through the indoor driving oil experimental evaluation of biological polymer flooding effect. The optimal bio polymer producing strain DJ was screened out. The strain had the ability to produce polymer and the product quantity was high. The effect of the biopolymer flooding was good. DJ strain optimum culture time for 72 hours, the optimal fermentation culture conditions: fermentation temperature 35 degrees Celsius; shaking speed r/min; 10 mL of inoculum; fermentation culture medium formula: glucose 40 g/L, 20 g/L of corn steep liquor, corn starch 2 G/L, NaNO3 3 g/L, KH2PO4 0.5 g/L, Na2HPO4·12H2O 1 g/L, MgSO4·7H2O 0.5 g/L, CaCl2 0.05 g/L, pH 7.2.