Endosymbiotic Actinidic Archaea and Viroidal Induced Warburg Phenotype Can be Reversed by a Modified Vegetarian High Fiber, High Medium Chain Triglyceride Ketogenic Diet

Abstract

Aims and Objectives: Actinidic archaea has been related to the pathogenesis of schizophrenia, malignancy, metabolic syndrome x, autoimmune disease and neuronal degeneration. The actinidic archaeal and viroid induced Warburg phenotype contributes to the pathology of the disease states mentioned. The possibility of administration of high medium chain triglyceride, high fiber vegetarian ketogenic diet on actinide based primitive organism like archaea with a mevalonate pathway and cholesterol catabolism was considered in these disease states. The effect of a high medium chain triglyceride and a high fiber modified vegetarian ketogenic diet on the Warburg phenotype was also studied. Methodology: The following groups were included in the study:- endomyocardial fibrosis, alzheimer’s disease, multiple sclerosis, non-hodgkin’s lymphoma, metabolic syndrome x with cerebrovascular thrombosis and coronary artery disease, schizophrenia, autism, seizure disorder, creutzfeldt jakob disease and acquired immunodeficiency syndrome. There were 10 patients in each group and each patient had an age and sex matched healthy control selected randomly from the general population. The blood was drawn from the (1) in freshly diagnosed cases and (2) after a 15-days modified vegetarian ketogenic diet of medium chain triglycerides (150 g of coconut oil), fiber (45 g of banana stem fiber) and vegetable proteins (black gram protein 100 g/day) with 50 g of carbohydrate (black gram polysaccharide). Plasma from fasting heparinised blood was used and the experimental protocol was as follows (I) Plasma+phosphate buffered saline, (II) same as I+cholesterol substrate, (III) same as II+rutile 0.1 mg/ml, (IV) same as II+ciprofloxacine and doxycycline each in a concentration of 1 mg/ml. The following estimations were carried out:- Cytochrome F420, free RNA, free DNA, hexokinase activity and archaeal cholesterol oxidase activity as indicated by liberation of hydrogen peroxide. Results: Plasma of control subjects showed increased levels of the above mentioned parameters with after incubation for 1 hour and addition of cholesterol substrate resulted in still further significant increase in these parameters. The plasma of patients showed similar results but the extent of increase was more. The addition of antibiotics to the control plasma caused a decrease in all the parameters while addition of rutile increased their levels. The addition of antibiotics and rutile to the patient’s plasma produced the same changes but the extent of change was more in patient’s sera as compared to controls. The patients on modified vegetarian high fiber, high medium chain triglyceride ketogenic diet showed a decrease in all the parameters. Conclusion: An actinide dependent shadow biosphere` of archaea and viroids in the above mentioned disease states is described. High dietary fiber and medium chain triglyceride ketogenic diet suppressed actinidic archaeal and viroidal growth, cholesterol oxidase activity and hexokinase activity. Thus the high fiber and medium chain triglyceride vegetarian ketogenic diet can inhibit the Warburg phenotype as well as has a antiarchaeal/viroidal effect.

Key words: Modified ketogenic diet; Medium chain triglyceride; Fiber; Archaea; Viroid; Warburg phenotype