Study of Mutagenesis on the Strain Producing Cellulase

Junhong LIU, Fumei WANG, Qi ZHAO, Hui CHENG

Abstract


Cellulose is insoluble in water, dilute acid and dilute alkali at room temperature, which is slowly decomposed under natural conditions. Microbial plays an important role in the degradation of cellulose. This article, taking the strain producing cellulase as the objective, dealed with the effect of mutagenesis on the activity of cellulase. The strain producing cellulase was activated and experienced mutagenesis by ultra violet, then fermentation experiment for enzyme production was carried out, DNS method was taken to calculate the activity of cellulase, hoping to find out optimum conditions for mutagenesis. It came to the conclusion that the activity of cellulase arrived at 107.75µg/mL·min on conditions of UV exposure for 4 min, accompanied by 34,600/mL growth concentration of strain.

Keywords


Cellulase; Enzyme Activity; Mutagenesis; Strain

Full Text:

PDF

References


[1] Wang, G. H., Tian, Y. L., & Yuan, J. T. (2005). Energy and Sustainable Development. Beijing: Chemical Industrial Press.

[2] Liu, B. Q., Li, X. F., Gao, W., & Yang, X. S. (2007). Study on Bioethanol Strain from Ignocellulose. Rewable Energy, 4, 53-55.

[3] Liu, J. H., & Yin, X. Z. (2010). Study on Optimized Conditions of Cellulose Degradation by Dilute Sulfuric Acid of Bermuda Grass. Rewable Energy, 8(4), 66-68.

[4] Bao, K., & Wang, X. H. (2007). Screening of Strain Producing Cellulase and Determination of Cellulase Activity. Journal of Biology, 24(2), 56-58.




DOI: http://dx.doi.org/10.3968/j.est.1923847920130501.658

DOI (PDF): http://dx.doi.org/10.3968/g3634

Refbacks

  • There are currently no refbacks.


Copyright (c)



Share us to:   

Reminder

If you have already registered in Journal A and plan to submit article(s) to Journal B, please click the CATEGORIES, or JOURNALS A-Z on the right side of the "HOME".

We only use three mailboxes as follows to deal with issues about paper acceptance, payment and submission of electronic versions of our journals to databases: [email protected]; [email protected]; [email protected]

 Articles published in Energy Science and Technology are licensed under Creative Commons Attribution 4.0 (CC-BY).

 ENERGY SCIENCE AND TECHNOLOGY Editorial Office

Address: 1055 Rue Lucien-L'Allier, Unit #772, Montreal, QC H3G 3C4, Canada.
Telephone: 1-514-558 6138 
Website: Http://www.cscanada.net Http://www.cscanada.org 
E-mail: [email protected]; [email protected]

Copyright © 2010 Canadian Research & Development Centre of Sciences and Cultures