Study of Mutagenesis on the Strain Producing Cellulase

Junhong LIU, Fumei WANG, Qi ZHAO, Hui CHENG

Abstract


Cellulose is insoluble in water, dilute acid and dilute alkali at room temperature, which is slowly decomposed under natural conditions. Microbial plays an important role in the degradation of cellulose. This article, taking the strain producing cellulase as the objective, dealed with the effect of mutagenesis on the activity of cellulase. The strain producing cellulase was activated and experienced mutagenesis by ultra violet, then fermentation experiment for enzyme production was carried out, DNS method was taken to calculate the activity of cellulase, hoping to find out optimum conditions for mutagenesis. It came to the conclusion that the activity of cellulase arrived at 107.75µg/mL·min on conditions of UV exposure for 4 min, accompanied by 34,600/mL growth concentration of strain.

Keywords


Cellulase; Enzyme Activity; Mutagenesis; Strain

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References


[1] Wang, G. H., Tian, Y. L., & Yuan, J. T. (2005). Energy and Sustainable Development. Beijing: Chemical Industrial Press.

[2] Liu, B. Q., Li, X. F., Gao, W., & Yang, X. S. (2007). Study on Bioethanol Strain from Ignocellulose. Rewable Energy, 4, 53-55.

[3] Liu, J. H., & Yin, X. Z. (2010). Study on Optimized Conditions of Cellulose Degradation by Dilute Sulfuric Acid of Bermuda Grass. Rewable Energy, 8(4), 66-68.

[4] Bao, K., & Wang, X. H. (2007). Screening of Strain Producing Cellulase and Determination of Cellulase Activity. Journal of Biology, 24(2), 56-58.




DOI: http://dx.doi.org/10.3968/j.est.1923847920130501.658

DOI (PDF): http://dx.doi.org/10.3968/g3634

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